Clarification of the C-terminal proteolytic processing site of human Amphiregulin.

نویسندگان

  • Kelly S Levano
  • Paraic A Kenny
چکیده

Amphiregulin, like other ErbB ligands, is synthesized as a pro-protein which requires cleavage at the cell surface to release the active signaling domain. Prior studies using a variety of approaches have not yielded a consensus about the precise cleavage site. Here we report the purification and protein sequencing of the cell-associated human Amphiregulin stalk which remains following cleavage of the signaling domain. These data indicate that human Amphiregulin is cleaved at Lysine 187, a site homologous to the cleavage site reported in the mouse protein and distinct from the Lysine 184 site previously reported for the human protein.

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عنوان ژورنال:
  • FEBS letters

دوره 586 19  شماره 

صفحات  -

تاریخ انتشار 2012